fret-based wild type rhoa biosensor vector Search Results


fret-based biosensor reporting on cdc42 activation  (Addgene inc)
90
Addgene inc fret-based biosensor reporting on cdc42 activation
Fret Based Biosensor Reporting On Cdc42 Activation, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rac1 generation fluorescence resonance energy transfer fret biosensor  (Addgene inc)
93
Addgene inc rac1 generation fluorescence resonance energy transfer fret biosensor
CD93 signaling activates <t>Rac1</t> at the cell edge of spreading cells. ( a ) <t>FRET</t> analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
Rac1 Generation Fluorescence Resonance Energy Transfer Fret Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rac1 generation fluorescence resonance energy transfer fret biosensor/product/Addgene inc
Average 93 stars, based on 1 article reviews
rac1 generation fluorescence resonance energy transfer fret biosensor - by Bioz Stars, 2026-04
93/100 stars
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ptriex rhoa biosensor wt  (Addgene inc)
92
Addgene inc ptriex rhoa biosensor wt
CD93 signaling activates <t>Rac1</t> at the cell edge of spreading cells. ( a ) <t>FRET</t> analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
Ptriex Rhoa Biosensor Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptriex rhoa biosensor wt/product/Addgene inc
Average 92 stars, based on 1 article reviews
ptriex rhoa biosensor wt - by Bioz Stars, 2026-04
92/100 stars
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flag p190b1rbs  (Addgene inc)
95
Addgene inc flag p190b1rbs
CD93 signaling activates <t>Rac1</t> at the cell edge of spreading cells. ( a ) <t>FRET</t> analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
Flag P190b1rbs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flag p190b1rbs/product/Addgene inc
Average 95 stars, based on 1 article reviews
flag p190b1rbs - by Bioz Stars, 2026-04
95/100 stars
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Image Search Results


CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.

Journal: International Journal of Molecular Sciences

Article Title: CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

doi: 10.3390/ijms222212417

Figure Lengend Snippet: CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.

Article Snippet: The following plasmids were purchased from Addgene (Watertown, MA, USA): a lentiviral negative control vector containing scrambled shRNA (#1864, sh-unr) [ ]; Rac1 generation fluorescence resonance energy transfer (FRET) biosensor for lentivirus production (#66111, pLenti-Rac1-2G) [ ]; FRET-based biosensor reporting on Cdc42 activation (#68813, pLenti-Cdc42-2G) [ ]; RhoA second-generation FRET biosensor for lentivirus production (#40179, pLenti-RhoA-2G) [ ]. shRNA-mediated knockdown of CD93 was performed as previously outlined [ ] by using a pLKO.1 retroviral vector from the Mission shRNA Library (Merck KGaA, Darmstadt, Germany), which expresses a shRNA (clone TRCN0000029085) specific for the silencing of the human protein.

Techniques: Transduction, Construct, Expressing, Fluorescence